Reporter groups on oligonucleotides


IBApy fluorophores

IBApy fluorophores span the complete visible spectrum and have spectral characteristics which are superior over commonly used dyes.
Advantages:
  • Higher quantum yields
  • Narrower emission bandwidths
  • Greater photostability
  • Insensitive to pH changes
  • Useful for DNA sequencing because the dye exhibits minimal effect on the mobility of the fragment during electrophoresis

Basic structure of the IBApy fluorophore


IBApy FL

The green fluorescent IBApy FL fluorophore has several characteristics that make it potentially superior to fluorescein.
These include:
  • High extinction coefficient
  • High fluorescent quantum yield (often approaching 1.0, even in water)
  • Spectral properties that are insensitive to solvent polarity and pH
  • Narrow emission bandwidth, resulting in a higher peak intensity than fluorescein
  • Little or no spectral overlap with longer wavelength dyes such as tetramethyl-rhodamine and Texas Red, making it useful for multicolor applications
  • Greater photostability than fluorescein in some environments
  • Lack of ionic charge
The different IBApy dyes are named according to their absorption and emission wave length or according to the dye they can substitute. The following IBApy fluorophores are available:

IBApy
absorption
emission
e [cm-1M-1]
493/503
500 nm
509 nm
79000
530/550
534 nm
551 nm
77000
FL (substitute for Fluorescein)
502 nm
510 nm
82000
R6G (substitute for Carboxyrhodamine 6G)
528 nm
547 nm
70000
TMR-X (substitute for TAMRA)
544 nm
570 nm
56000
Sold under license from Molecular Probes, Inc. (owned by Invitrogen Corp.)

All IBApy fluorophores are available in the following scales:

Order information
see also "how to order"

order online Order online

product
scale
amount
cat. no.
price/coupling
5'-IBApy
0.05 µmol
5 nmol
5-0310-122
79.00 EUR
0.20 µmol
25 nmol
5-0310-123
94.00 EUR
1.00 µmol
100 nmol
5-0310-124
165.00 EUR
3'-IBApy
0.05 µmol
5 nmol
5-0310-102
79.00 EUR
0.20 µmol
25 nmol
5-0310-103
94.00 EUR
1.00 µmol
100 nmol
5-0310-104
165.00 EUR
internal IBApy
0.05 µmol
5 nmol
5-0310-112
100.00 EUR
0.20 µmol
25 nmol
5-0310-113
115.00 EUR
1.00 µmol
100 nmol
5-0310-114
165.00 EUR



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