Reporter Groups on Oligonucleotides

• Biotin
  
• Imminobiotin
  
• Desthiobiotin
• Oligo (dT)20
  
• Dig
  

Biotin, Iminobiotin and Digoxigenin labeled oligonucleotides

• Labeled probes can be stored for a long period without loss of label or activity
• Hybridization solutions can be reused several times
• The technology is non-hazardous
• A wide range of enzyme conjugates are available for detection and signal amplification (dye, fluorescence, luminescence)

Biotin

Biotin is a very popular hapten marker for oligonucleotides. An extensive variety of products are available for the detection and immobilization of biotin labeled probes. As biotin has a high affinity to streptavidin, the detection of biotin labeled probes is very sensitive. Examples of this are in situ hybridization, cDNA synthesis with biotin oligo-dT, capture of amplified DNA sequences and solid phase sequencing. Biotin can be introduced either at the 3'-end and/or at the 5'-end and at internal sites at any thymidine residue. For best results and highest sensitivity the distance between each biotin in the probe should be at least 15 bases. Every biotin labeled oligonucleotide is carefully analyzed by HPLC for quantitative labeling.

Now also available for Click-Chemistry!

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product	scale	amount	cat. no.	price/coupling
5'-Biotin	0.05 µmol	5 nmol	5-0320-032	38.50 EUR
	0.20 µmol	25 nmol	5-0320-033	48.50 EUR
	1.00 µmol	100 nmol	5-0320-034	100.00 EUR
3'-Biotin	0.05 µmol	5 nmol	5-0320-012	48.50 EUR
	0.20 µmol	25 nmol	5-0320-013	64.00 EUR
	1.00 µmol	100 nmol	5-0320-014	115.00 EUR
Internal Biotin	0.05 µmol	5 nmol	5-0320-022	90.00 EUR
	0.20 µmol	25 nmol	5-0320-023	100.00 EUR
	1.00 µmol	100 nmol	5-0320-024	150.00 EUR

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Iminobiotin

Mild purification conditions for active reporter molecules in e.g. DNA/DNA or DNA/protein binding studies

2-Iminobiotin, an analogue of biotin, interacts with avidin and streptavidin in a pH-dependent manner allowing recovery of your reporter molecule coupled to iminobiotin. At high pH, the free base form of 2-iminobiotin retains the high-affinity specific binding characteristics of biotin whereas at acidic pH values the salt form of the analogue interacts poorly with avidin. Using these properties for an affinity purification protocol you will be able to load your biomolecule to an avidin matrix at pH 9.5 and elute it at pH 4, thus, overcoming the harsh elution conditions associated with biotin.

We offer

• oligonucleotides labeled with iminobiotin or biotin
  
• carefully analyzed by HPLC for quantitative labeling
  
• labels introduced at the 3´-end, 5´-end and internally at any thymidine residue
  
  

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product	amount	cat. no.	price/coupling
5´-Iminobiotin	0.05 µmol	5-0322-032	48.00 EUR
	0.20 µmol	5-0322-033	64.00 EUR
	1.00 µmol	5-0322-034	100.00 EUR
3´-Iminobiotin	0.05 µmol	5-0322-012	58.00 EUR
	0.20 µmol	5-0322-013	74.00 EUR
	1.00 µmol	5-0322-014	115.00 EUR
Internal Iminobiotin	0.05 µmol	5-0322-022	100.00 EUR
	0.20 µmol	5-0322-023	115.00 EUR
	1.00 µmol	5-0322-024	160.00 EUR

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Desthiobiotin

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product	amount	cat. no.	price/coupling
5´-Desthiobiotin	0.05 µmol	5-0322-062	48.00 EUR
	0.20 µmol	5-0322-063	64.00 EUR
	1.00 µmol	5-0322-064	100.00 EUR
3´-Desthiobiotin	0.05 µmol	5-0322-042	58.00 EUR
	0.20 µmol	5-0322-043	74.00 EUR
	1.00 µmol	5-0322-044	115.00 EUR
Internal Desthiobiotin	0.05 µmol	5-0322-052	100.00 EUR
	0.20 µmol	5-0322-053	115.00 EUR
	1.00 µmol	5-0322-054	160.00 EUR

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Oligo (dT)₂₀ Probe, biotin-labeled

Probe for immobilization of mRNA on streptavidin-coated solid supports

This biotin-labeled oligo (dT) probe can be hybridized with eukaryotic mRNA for subsequent immobilization on streptavidin-coated surfaces, such as PCR tubes or magnetic beads. For further downstream applications like RT-PCR the immobilized mRNA can be isolated or directly processed.

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product	amount	cat. no.	price/coupling
Oligo (dT)20 Probe, biotin-labeled, HPLC purified	25 nmol	5-0000-303	55.00 EUR
	100 nmol	5-0000-304	115.00 EUR

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Digoxigenin

Digoxigenin (Dig*) is a hapten occuring naturally only in Digitalis purpurea. This explains the low endogenous background associated in applications with Dig. A range of conjugated anti-Dig antibodies are available, such as alkaline phosphatase, peroxidase, fluorescein and rhodamine. Dig can be introduced either at the 3'-end and/or the 5'-end as well as at internal sites at any thymidine residue. For best results and highest sensitivity the distance between each Dig in the probe should be at least 15 bases.
Every Dig labeled oligonucleotide is carefully analyzed by HPLC for quantitative labeling.

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product	scale	amount	cat. no.	price/coupling
5'-Dig	0.05 µmol	5 nmol	5-0321-032	48.50 EUR
	0.20 µmol	25 nmol	5-0321-033	64.00 EUR
	1.00 µmol	100 nmol	5-0321-034	115.00 EUR
3'-Dig	0.05 µmol	5 nmol	5-0321-012	64.00 EUR
	0.20 µmol	25 nmol	5-0321-013	79.00 EUR
	1.00 µmol	100 nmol	5-0321-014	130.00 EUR
Internal Dig	0.05 µmol	5 nmol	5-0321-022	90.00 EUR
	0.20 µmol	25 nmol	5-0321-023	100.00 EUR
	1.00 µmol	100 nmol	5-0321-024	150.00 EUR

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see also "how to order"